Products Included | No. | Volume | Applicaton | Dilution | Reactivity | Homology† |
---|---|---|---|---|---|---|
PathScan® EMT Duplex IF Kit Primary Antibody Cocktail | 7783 | 100 µl | Immunofluorescence (Immunocytochemistry), Immunofluorescence (Paraffin) | 1:100 | Human | Mouse |
PathScan® Duplex IF Kit Detection Cocktail I | 7832 | 100 µl | Immunofluorescence (Immunocytochemistry), Immunofluorescence (Paraffin) | 1:100 | N/A | N/A |
†Species predicted to react based on 100% sequence homology.
Kit Analytes | Detection Dye | Ex(max) (nm) | Em(max) (nm) |
---|---|---|---|
E-cadherin | Alexa Fluor® 488 | 495 | 519 |
Vimentin | Alexa Fluor® 555 | 555 | 565 |
IF-P, IF-IC
#P08670, #P12830
7431, 999
Product Information
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein or residues surrounding Pro780 of human E-cadherin protein.
Product Description
Background
Epithelial-mesenchymal transition (EMT) refers to a biological process in which cells undergo a series of biochemical changes that induce a morphological transformation from an epithelial, polarized, adhesive state to an irregular, elongated, mesenchymal phenotype that enables migratory capacity (1,2). EMTs are classified into three subtypes: those involved in implantation, embryogenesis, and organ development; those associated with inflammation and fibrosis; and those involved in invasion and metastasis (1). Molecular changes that are associated with cells during this transformation include the loss of E-cadherin and gain of vimentin expression, hallmark epithelial and mesenchymal markers, respectively (3-5). Numerous studies have established that EMT is an essential step in cancer metastasis (5-7). E-cadherin is regarded as an active suppressor of invasion and tumorigenesis (8). In response to extracellular stimuli, vimentin coordinates various signaling pathways to induce spatial reorganization and structural changes (9), reminiscent of the EMT phenotype observed in motile cells involved in invasion and metastasis (6).
- Kalluri, R. and Weinberg, R.A. (2009) J Clin Invest 119, 1420-8.
- Lee, J.M. et al. (2006) J Cell Biol 172, 973-81.
- Yan, W. et al. (2010) J Biol Chem 285, 14042-51.
- Sethi, S. et al. (2011) Transl Oncol 4, 222-6.
- Kim, J.H. et al. (2007) J Korean Med Sci 22, 898-904.
- Emadi Baygi, M. et al. (2010) Cell Biol Toxicol 26, 553-67.
- Sethi, S. et al. (2010) Am J Transl Res 3, 90-9.
- Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
- Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-P: Immunofluorescence (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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