WB, IP, IHC-P
H M R
Endogenous
150
Rabbit IgG
#Q14203
1639
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 |
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1206 of human dynactin p150Glued protein.
Background
Cytoplasmic dynein is a multi-subunit motor complex that regulates microtubule organization as well as the transport and positioning of organelles. Dynactin is a multi-subunit dynein-activating complex, which regulates the interaction of the dynein motor with various cellular cargoes, and enhances dynein’s processivity. p150Glued/DCTN1/Dynactin 1 is the largest subunit of the dynactin complex (1-3). In mitosis, cytoplasmic dynein regulates spindle organization, chromosome movement and centrosome separation (4). The dynactin subunit p150Glued is phosphorylated at serine 19 by the mitotic kinase aurora A during anaphase, and this phosphorylation is required for the appropriate regulation of spindle assembly (5). In neurons, axonal transport is important for cellular function and survival. Dysfunction and mutations in dynein and dynactin subunits, including p150Glued, have been linked to human neurodegenerative diseases such as Alzheimer’s Disease (6-7), Perry Syndrome (8) and ALS (9).
- Gill, S.R. et al. (1991) J Cell Biol 115, 1639-50.
- McGrail, M. et al. (1995) J Cell Biol 131, 411-25.
- Plamann, M. et al. (1994) J Cell Biol 127, 139-149.
- Raaijmakers, J.A. and Medema, R.H. (2014) Chromosoma 123, 407-22.
- Reboutier, D. et al. (2013) J Cell Biol 201, 65-79.
- Fujiwara, T. et al. (2012) J Neurochem 122, 162-74.
- Kimura, N. et al. (2016) Am J Pathol , .
- Araki, E. et al. (2014) Mov Disord 29, 1201-4.
- Stockmann, M. et al. (2013) J Neural Transm (Vienna) 120, 785-98.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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