IHC-Bond, IHC-P
H
Endogenous
Mouse IgG2b
#Q5XXA6
55107
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the carboxy terminus of human DOG1/Anoctamin 1 protein.
Background
Anoctamin 1, also called DOG1 for discovered on gastrointestinal stromal tumors protein 1 or TMEM16A for Transmembrane Member 16A, is a plasma membrane calcium-activated chloride channel (1). It is essential for chloride secretion from epithelial tissues (2), and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified Anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5).
- Schroeder, B.C. et al. (2008) Cell 134, 1019-29.
- Thomas-Gatewood, C. et al. (2011) Am J Physiol Heart Circ Physiol 301, H1819-27.
- Gomez-Pinilla, P.J. et al. (2009) Am J Physiol Gastrointest Liver Physiol 296, G1370-81.
- Hwang, S.J. et al. (2009) J Physiol 587, 4887-904.
- Cho, H. et al. (2012) Nat Neurosci 15, 1015-21.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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