WB, IP
H M R
Endogenous
215
Rabbit
#Q14185
1793
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human DOCK180.
Background
DOCK180 and its partner, ELMO1, interact directly with one another to form an atypical two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). Rac activation occurs in association with p130 Cas and Crk, which form a complex with DOCK180 that is targeted to focal adhesions (1,2). DOCK180 is also recruited to the plasma membrane by binding to phosphoinositides (3). ELMO1 may function as an inhibitor of proteasome-dependent degradation of DOCK180 at the plasma membrane to regulate reorganization of the actin cytoskeleton (4). Localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes such as phagocytosis and cell migration (5-7).
- Brugnera, E. et al. (2002) Nat. Cell Biol. 4, 574-582.
- Matsuda, M. et al. (1996) J. Biol. Chem. 271, 14468-14472.
- Côté, J.F. et al. (2005) Nat. Cell Biol. 7, 797-807.
- Makino, Y. et al. (2006) J. Cell Sci. 119, 923-932.
- Takenawa, T. and Miki, H. (2001) J. Cell Sci. 114, 1801-1809.
- Albert, M.L. et al. (2000) Nat. Cell Biol. 2, 899-905.
- Gustavsson, A. et al. (2004) J. Biol. Chem. 279, 22893-22901.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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