DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb (BSA and Azide Free) (#10208) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IHC-P

REACTIVITY:

H M R

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#O15075

Entrez-Gene Id:

9201

Product Information

Product Usage Information

This product is the carrier free version of product #62257. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #62257

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

DCLK1/DCAMKL1 (D2U3L) XP^® Rabbit mAb recognizes endogenous levels of total DCLK1/DCAMKL1 protein. The identity of the faint lower molecular weight bands is not known.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DCLK1/DCAMKL1 protein.

Background

Doublecortin-like kinase 1 (DCLK1, DCAMKL1) is a serine/threonine kinase that belongs to the CaM kinase family and shares homology with the neuronal microtubule binding protein doublecortin. DCLK1 is thought to be involved in calcium signaling pathways controlling neuronal development in the embryonic brain (1,2). The kinase also functions in the mature nervous system and is highly expressed in regions of active neurogenesis in the neocortex and cerebellum (3,4). Research studies suggest that the DCLK1 kinase is highly expressed in subpopulations of cells within the colon and gastric epithelium and in the pancreas (5-8). The nature of these cell populations, whether normal, stem-like, or tumor-initiating, is unclear.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a registered trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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