Cyclin C (E6V4Z) Rabbit mAb (#68179) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, IF-IC, FC-FP

REACTIVITY:

H M

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P24863

Entrez-Gene Id:

892

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunofluorescence (Immunocytochemistry)	1:1600 - 1:6400
Flow Cytometry (Fixed/Permeabilized)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cyclin C (E6V4Z) Rabbit mAb recognizes endogenous levels of total cyclin C protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human cyclin C protein.

Background

Cyclin dependent kinases (CDKs) are regulated in part through binding to regulatory cyclins. While many cyclin/CDK complexes regulate progression through the cell cycle, others, such as cyclin C/CDK8, regulate transcription and other cellular functions. Cyclin C/CDK8 forms a regulatory sub-complex with Med12 and Med13, which associates with the multi-subunit transcriptional coactivator Mediator Complex to modulate RNA polymerase II-dependent transcription. In addition to its role in transcription, cyclin C is important in the regulation of mitochondrial dynamics, apoptosis, and cell cycle progression (1). Cytosolic cyclin C induces mitochondrial fragmentation and apoptosis, and is required for activation of the Bcl2 family member, Bax (2). Cytoplasmic cyclin C also supports mitochondrial fission through dynamin-related protein 1 (Drp1) in response to oxidative stress (3). Cyclin C acts synergistically with PTEN to maintain levels of tumor suppressors p53 and p21 in a mouse model of thyroid cancer (4). In complex with CDK3, cyclin C regulates Rb-dependent exit from quiescence in human cells (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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