| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| Cyclin A2 (BF683) Mouse mAb | 4656 | 20 µl | 55 kDa | Mouse IgE |
| Cyclin B1 Antibody | 4138 | 20 µl | 55 kDa | Rabbit |
| Cyclin D1 (92G2) Rabbit mAb | 2978 | 20 µl | 36 kDa | Rabbit IgG |
| Cyclin D2 (D52F9) Rabbit mAb | 3741 | 20 µl | 31 kDa | Rabbit IgG |
| Cyclin D3 (DCS22) Mouse mAb | 2936 | 20 µl | 31 kDa | Mouse IgG1 |
| Cyclin E1 (HE12) Mouse mAb | 4129 | 20 µl | 48 kDa | Mouse IgG1 |
| Cyclin E2 Antibody | 4132 | 20 µl | 48 kDa | Rabbit |
| Cyclin H Antibody | 2927 | 20 µl | 36 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.
Storage
Background
Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).
- Krempler, A. et al. (2005) Cell Mol Life Sci 62, 1379-87.
- Toyoshima, F. et al. (1998) EMBO J 17, 2728-35.
- Porter, L.A. and Donoghue, D.J. (2003) Prog Cell Cycle Res 5, 335-47.
- Benzeno, S. et al. (2006) Oncogene 25, 6291-303.
- Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.
- Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
- Sherr, C.J. (1996) Science 274, 1672-7.
- Kim, J.K. and Diehl, J.A. (2009) J Cell Physiol 220, 292-6.
- Zacharek, S.J. et al. (2005) Cancer Res 65, 11354-60.
Background References
Trademarks and Patents
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
