WB, IP
H
Endogenous
15
Rabbit IgG
#Q07325
4283
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CXCL9/MIG protein.
Background
C-X-C motif chemokine ligand 9 (CXCL9, MIG) is a soluble chemokine expressed by multiple cell types, such as endothelial cells, monocytes, and tumor cells. The expression of CXCL9 is upregulated in response to proinflammatory cytokines, such as IFN-γ and TNF-α. (1,2). CXCL9 binds to CXCR3, a GPCR that is expressed on the surface of multiple populations of immune cells, particularly exclusively activated T lymphocytes (3). Binding of CXCL9 to CXCR3 promotes T cell chemotaxis and infiltration into sites of inflammation that form in settings such as viral infection and tumorigenesis (4-6). Research studies have demonstrated production of CXCL9 by tumor cells mediates tumor suppression through the recruitment of tumor antigen-specific T cells (7).
- Shin, S.Y. et al. (2010) J Biol Chem 285, 30731-40.
- Hiroi, M. and Ohmori, Y. (2003) Biochem J 376, 393-402.
- Loetscher, M. et al. (1996) J Exp Med 184, 963-9.
- Tensen, C.P. et al. (1999) J Invest Dermatol 112, 716-22.
- Han, K. et al. (2020) Am J Respir Cell Mol Biol, doi: 10.1165/rcmb.2020-0354OC.
- Russo, E. et al. (2020) J Leukoc Biol 108, 673-685.
- Gorbachev, A.V. et al. (2007) J Immunol 178, 2278-86.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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