WB
H
Endogenous
10
Rabbit IgG
#P02778
3627
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human CXCL10 protein.
Background
Chemokine (C-X-C motif) ligand 10 (CXCL10, IP-10) is a chemokine produced by a number of cell types and that is induced in response to pro-inflammatory cytokines, such as IFN-γ and TNF-α (1,2). CXCL10 binds to the chemokine receptor CXCR3 and induces calcium flux and cell migration (3). The CXCR3 protein is normally expressed on activated CD4+ T cells and NK cells, which can produce pro-inflammatory cytokines, including IFN-γ and TNF-α (3,4). As a result, these molecules act in an amplification loop to promote immune responses in the contexts of both autoimmunity and a tumor immune response (5,6).
- Shin, S.Y. et al. (2010) J Biol Chem 285, 30731-40.
- Luster, A.D. and Ravetch, J.V. (1987) J Exp Med 166, 1084-97.
- Loetscher, M. et al. (1996) J Exp Med 184, 963-9.
- Kajitani, K. et al. (2012) Breast Cancer Res Treat 134, 139-55.
- Luster, A.D. and Leder, P. (1993) J Exp Med 178, 1057-65.
- Antonelli, A. et al. (2014) Autoimmun Rev 13, 272-80.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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