WB, IP
H
Endogenous
200
Rabbit
#P39880
1523
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln639 of human CUTL1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CUTL1 (Cut-like 1), also known as CUX1 (Cut homeobox 1) (CUX1), is a transcription factor that has been implicated in many cellular processes in different tissues, such as cell migration, neuronal differentiation, and DNA repair (1-5). CUTL1 expression and activities are altered in cancer. Research studies have shown the CUTL1 gene to be a frequent target of loss-of-heterozygocity in various cancers (6,7). On the other hand, CUTL1 expression is elevated in many cancers and is associated with shorter disease-free survival (8). These accumulating evidence suggest that decreased CUTL1 expression promote tumor initiation and increased CUTL1 expression facilitates tumor progression (9). While full-length CUTL1 is about 200 kDa (p200), short forms p110 and p75 can also be generated by proteolytic processing and alternative transcription initiation site, respectively (10, 11).
- Rodríguez-Tornos, F.M. et al. (2016) Neuron 89, 494-506.
- Ramdzan, Z.M. et al. (2015) Oncotarget 6, 3613-26.
- Ramdzan, Z.M. et al. (2014) PLoS Biol 12, e1001807.
- Kedinger, V. et al. (2009) J Biol Chem 284, 27701-11.
- Vadnais, C. et al. (2012) Nucleic Acids Res 40, 4483-95.
- McNerney, M.E. et al. (2013) Blood 121, 975-83.
- Ramdzan, Z.M. and Nepveu, A. (2014) Nat Rev Cancer 14, 673-82.
- Michl, P. et al. (2005) Cancer Cell 7, 521-32.
- Hulea, L. and Nepveu, A. (2012) Gene 497, 18-26.
- Moon, N.S. et al. (2001) Mol Cell Biol 21, 6332-45.
- Goulet, B. et al. (2002) Cancer Res 62, 6625-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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