WB, IP
H Mk
Endogenous
50
Rabbit IgG
#P35790
1119
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro85 of human choline kinase α protein.
Background
Choline kinase (ChoK) catalyzes the phosphorylation of choline, a key step in the biosynthesis of the membrane phospholipid phosphatidylcholine. At least three ChoK isoforms exist in mammalian cells, α-1, α-2, and β. The two α isoforms are transcribed from the same CHKA gene as splice variants, while the β isoform resides on a separate CHKB gene (reviewed in 1).
Research studies indicate that ChoKα levels affect signaling through MAPK and Akt pathways (2,3). Investigators have shown that ChoKα plays a role in proliferation and carcinogenesis and is highly expressed/activated in human cancers (4-7). Additional research studies suggest ChoKα may be a potential target for cancer therapy (8).
- Janardhan, S. et al. (2006) Curr Med Chem 13, 1169-86.
- Yalcin, A. et al. (2010) Oncogene 29, 139-49.
- Chua, B.T. et al. (2009) Mol Cancer 8, 131.
- Ramírez de Molina, A. et al. (2002) Oncogene 21, 4317-22.
- Ramírez de Molina, A. et al. (2007) Lancet Oncol 8, 889-97.
- Hernando, E. et al. (2009) Oncogene 28, 2425-35.
- Miyake, T. and Parsons, S.J. (2012) Oncogene 31, 1431-41.
- Bañez-Coronel, M. et al. (2008) Curr Cancer Drug Targets 8, 709-19.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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