WB
H
Endogenous
270
Rabbit
#Q02224
1062
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1897 of human CENP-E protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Centromere-associated protein E (CENP-E) is a kinesin-like motor protein and mitotic-checkpoint kinase BUB1B binding partner that is essential for establishing and maintaining stable attachments between mitotic chromosomes and spindle microtubules (1). CENP-E plays an important role as a motor protein in the alignment of chromosomes during prometaphase (2). Research studies indicate that CENP-E protein expression peaks in late G2 and M-phases of the cell cycle before the protein is degraded at mitotic exit (3). Additional studies show that the loss of CENP-E function results in cell cycle arrest in mitosis. Mutations in the corresponding CENPE gene can result in autosomal recessive primary microcephaly-13, a developmental disorder characterized by small head circumference, dysmorphic facial features, short stature, and delayed psychomotor development (4). Since CENP-E is essential for mitotic progression and is required for cellular proliferation, it has become an interesting target for cancer therapy (5-7).
- Yao, X. et al. (2000) Nat Cell Biol 2, 484-91.
- Yardimci, H. et al. (2008) Proc Natl Acad Sci U S A 105, 6016-21.
- Brown, K.D. et al. (1994) J Cell Biol 125, 1303-12.
- Mirzaa, G.M. et al. (2014) Hum Genet 133, 1023-39.
- Wood, K.W. et al. (2008) Clin Cancer Res 14, 7588-92.
- Balamuth, N.J. et al. (2010) Cancer Res 70, 2749-58.
- Kung, P.P. et al. (2014) Mol Cancer Ther 13, 2104-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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