CDK2AP1 (E9D7P) Rabbit mAb (#89650) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IP, ChIP

REACTIVITY:

H Mk

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#O14519

Entrez-Gene Id:

8099

Product Information

Product Usage Information

For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10⁶ cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Chromatin IP	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CDK2AP1 (E9D7P) Rabbit mAb recognizes endogenous levels of total CDK2AP1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human CDK2AP1 protein.

Background

Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was first identified as a tumor suppressor in oral cancer and is a member of the nucleosome remodeling and histone deacetylase (NuRD) complex (1-4). Also known as p12 or DOC-1, CDK2AP1 binds to unphosphorylated CDK2 and DNA polymerase alpha/primase to negatively regulate their activities (5,6). CDK2AP1 also plays a role in the silencing of Oct-4 during stem cell differentiation (7). Low CDK2AP1 expression in oral cancers is associated with poor prognosis (8). Lower expression has also shown to be resistant to cisplatin treatment (9). In addition to oral cancers, CDK2AP1 plays a role in other tumor types, including colorectal, testicular, and ovarian cancers (10,11).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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