WB, IP, IHC-P, FC-L
H
Endogenous
120, 82
Rabbit IgG
#Q9NPY3
22918
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
| Flow Cytometry (Live) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #55570.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD93/C1qR protein.
Background
CD93/complement component C1q receptor (C1qR) is a type-I transmembrane glycoprotein belonging to the CLEC14 family of surface antigens, which share a similar ectodomain architecture highlighted by the presence of a C-type lectin domain and multiple EGF-like domains. CD93/C1qR expression is primarily restricted to endothelial cells associated with normal and tumor-associated vasculature as well as immune cells of the myeloid lineage, including monocytes and neutrophils (1). The extracellular domain of CD93/C1qR is rich in O-linked glycosylation modifications, which stabilizes its surface expression by protecting it from proteolytic cleavage (2,3). CD93/C1qR regulates various aspects of endothelial cell biology, including proliferation, migration, and adhesion (4). Indeed, research studies have demonstrated that surface expressed CD93/C1qR binds to an endothelial specific extracellular matrix ligand, multimerin-2, and disrupting the interaction compromises angiogenesis (5). Furthermore, CD93 expression is upregulated in tumor vasculature and correlates with poor survival (6-8).
- Nepomuceno, R.R. and Tenner, A.J. (1998) J Immunol 160, 1929-35.
- Park, M. and Tenner, A.J. (2003) J Cell Physiol 196, 512-22.
- Ikewaki, N. et al. (2013) Microbiol Immunol 57, 822-32.
- Orlandini, M. et al. (2014) Oncotarget 5, 2750-60.
- Khan, K.A. et al. (2017) Oncogene 36, 6097-6108.
- Bao, L. et al. (2016) Biochem Biophys Res Commun 476, 467-474.
- Langenkamp, E. et al. (2015) Cancer Res 75, 4504-16.
- Sun, Y. et al. (2021) Sci Transl Med 13, eabc8922. doi: 10.1126/scitranslmed.abc8922.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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