WB, W-S
H
Endogenous
22, 24, 35
Rabbit IgG
#P21926
928
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val178 of human CD9 protein.
Background
The CD9 antigen belongs to the tetraspanin family of cell surface glycoproteins, and is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs), where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (1). Research studies demonstrate that CD9 expression on the egg is required for gamete fusion during fertilization (2-4). CD9 was also shown to play a role in dendritic cell migration, megakaryocyte differentiation, and homing of cord blood CD34+ hematopoietic progenitors to the bone marrow (5-7). In addition, downregulation of CD9 expression is associated with poor prognosis and progression of several types of cancer (8-10). Additional research identified CD9 as an abundant component of exosomes, and may play some role in the fusion of these secreted membrane vesicles with recipient cells (11).
- Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
- Le Naour, F. et al. (2000) Science 287, 319-21.
- Miyado, K. et al. (2000) Science 287, 321-4.
- Kaji, K. et al. (2000) Nat Genet 24, 279-82.
- Mantegazza, A.R. et al. (2004) Blood 104, 1183-90.
- Clay, D. et al. (2001) Blood 97, 1982-9.
- Leung, K.T. et al. (2011) Blood 117, 1840-50.
- Miyake, M. et al. (1995) Cancer Res 55, 4127-31.
- Higashiyama, M. et al. (1995) Cancer Res 55, 6040-4.
- Uchida, S. et al. (1999) Br J Cancer 79, 1168-73.
- Théry, C. et al. (1999) J Cell Biol 147, 599-610.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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