WB, IP
M
Endogenous
45-80
Rabbit
#Q18PI6
12523
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala322 of mouse CD84 protein. Antibodies are purified by peptide affinity chromatography.
Background
Cluster of Differentiation 84 (CD84), also known as signaling lymphocytic activation molecule family member 5 (SLAMF5), is a membrane glycoprotein expressed by numerous immune cell types, including hematopoietic progenitor cells, T cells, B cells, NK cells, macrophages, monocytes, dendritic cells, platelets, mast cells, and thymocytes (1-9). CD84 is a homophilic adhesion molecule involved in modulating the activation, differentiation, proliferation, and cytotoxicity of a wide variety of immune cells, and thus is involved in the regulation and interconnection of both the innate and adaptive immune response (6-14). Certain CD84 activities are regulated by the presence or absence of small cytoplasmic adaptor proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (5,14). Alternate splicing results in multiple transcript variants.
- Zaiss, M. et al. (2003) Exp Hematol 31, 798-805.
- de la Fuente, M.A. et al. (1997) Blood 90, 2398-405.
- Krause, S.W. et al. (2000) Biochem J 346 Pt 3, 729-36.
- Romero, X. et al. (2004) Tissue Antigens 64, 132-44.
- Tangye, S.G. et al. (2002) Eur J Immunol 32, 1640-9.
- Álvarez-Errico, D. et al. (2011) J Immunol 187, 5577-86.
- Nanda, N. et al. (2005) Blood 106, 3028-34.
- Tangye, S.G. et al. (2003) J Immunol 171, 2485-95.
- Martin, M. et al. (2001) J Immunol 167, 3668-76.
- Sintes, J. et al. (2010) J Leukoc Biol 88, 687-97.
- Wong, E.B. et al. (2015) J Immunol 194, 4130-43.
- Agod, Z. et al. (2018) Front Immunol 9, 62.
- Cannons, J.L. et al. (2010) Immunity 32, 253-65.
- Wang, N. et al. (2010) J Immunol 185, 5683-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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