CD68 (E3O7V) Rabbit mAb (#97778) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, IHC-Bond, IHC-P, IF-F, IF-IC, FC-FP, FC-L

REACTIVITY:

M R Hm

SENSITIVITY:

Endogenous

MW (kDa):

70-80, 130-140, 200

Source/Isotype:

Rabbit IgG

UniProt ID:

#P31996

Entrez-Gene Id:

12514

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
IHC Leica Bond	1:600 - 1:2400
Immunohistochemistry (Paraffin)	1:150 - 1:600
Immunofluorescence (Frozen)	1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)	1:200 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:50 - 1:200
Flow Cytometry (Live)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #29176.

Specificity / Sensitivity

CD68 (E3O7V) Rabbit mAb recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

Species Reactivity:

Mouse, Rat, Hamster

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

Background

CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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