Revision 8
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IHC-P, IF-IC, FC-FP, FC-L

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P34810

Entrez-Gene Id:

968

Product Information

Product Usage Information

This product is the carrier free version of product #76437. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #76437.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD68 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CD68 protein.

Background

CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).

  1. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  2. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  3. Ramprasad, M.P. et al. (1995) Proc Natl Acad Sci U S A 92, 9580-4.
  4. Ramprasad, M.P. et al. (1996) Proc Natl Acad Sci U S A 93, 14833-8.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Revision 8
#26042

CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemistry Image 1: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® Rabbit mAb 对石蜡包埋的人扁桃体进行免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunohistochemistry Image 2: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® Rabbit mAb 对石蜡包埋的人子宫浆液性乳头状癌进行免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunohistochemistry Image 3: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® Rabbit mAb 对石蜡包埋的人肺癌进行免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunohistochemistry Image 4: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® Rabbit mAb 对石蜡包埋的正常食蟹猴脾脏进行免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunohistochemistry Image 5: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® rabbit mAb(红色)、PD-1 (EH33) mouse mAb #43248(绿色)、CD8α (C8/144B) mouse mAb #70306(品红色)、Pan-keratin (C11) mouse mAb #4545(青色)、LAG3 (D2G4O) XP® rabbit mAb #15372(橙色)和 TIM-3 (D5D5R) XP® rabbit mAb #45208(黄色)对石蜡包埋的人肺腺癌进行多重免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunohistochemistry Image 6: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® rabbit mAb(橙色)、PD-L1 (E1L3N®) XP® rabbit mAb #13684(红色)、PD-L2 (D7U8C™) XP® 兔单克隆抗体 #(品红色)、PD-L2 (D7U8C) XP® rabbit mAb (magenta) #82723, Arginase-1 (D4E3M) XP® rabbit mAb #93668(绿色)、IDO (D5J4E) rabbit mAb #86630(黄色)和 Pan-keratin (C11) mouse mAb #4545(青色)对石蜡包埋的人卵巢癌进行多重免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunohistochemistry Image 7: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® Rabbit mAb 对石蜡包埋的 THP-1 细胞沉淀物(左)和 Jurkat 细胞沉淀物(右)进行免疫组织化学分析。数据的生成使用的是该产品的常规组分产品。
Immunofluorescence Image 1: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) XP® Rabbit mAb(绿色)和 DRAQ5® #4084 (fluorescent DNA dye)(蓝色)对 THP-1 细胞(左图,阳性)和 Jurkat 细胞(右图,阴性)进行共聚焦免疫荧光分析。显示来自 z-stack 系列的投射图像。采用本产品的标准制剂生成数据。
Flow Cytometry Image 1: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
以浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 作为对照,使用 CD68 (D4B9C) XP® Rabbit mAb(右图)对用抗人 CD14 共染色的活性人外周血单核细胞进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 用作二抗。数据的生成使用的是该产品的常规组分产品。
Flow Cytometry Image 2: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
以浓度匹配的兔 (DA1E) 单抗 IgG XP® 同型对照 #3900(左图)作为对照,使用 CD68 (D4B9C) XP® 兔单抗(右图)对用抗人 CD14 共染色的固定和透化人外周血单核细胞进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 用作二抗。数据的生成使用的是该产品的常规组分产品。
Flow Cytometry Image 3: CD68 (D4B9C) XP® Rabbit mAb (BSA and Azide Free) Expand Image
使用 CD68 (D4B9C) Rabbit mAb(实线)或浓度匹配的 Rabbit (DA1E) mAb IgG XP® Isotype Control #3900(虚线)对固定和通透的 Jurkat 细胞(蓝色,阴性)和 THP-1 细胞(绿色,阳性)进行流式细胞分析。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 用作二抗。数据的生成使用的是该产品的常规组分产品。