CD64/FcγRI (E2O4L) Rabbit mAb (#90694) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

60-80

Source/Isotype:

Rabbit IgG

UniProt ID:

#P26151

Entrez-Gene Id:

14129

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD64/FcγRI (E2O4L) Rabbit mAb recognizes endogenous levels of total CD64/FcγRI protein. This antibody does not cross-react with mouse FcγRIV, FcγRIIA, or FcγRIIB.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile256 of mouse CD64/FcγRI protein.

Background

CD64/FcγRI (high affinity IgG Fc gamma RI) is an activating Fcγ receptor in the immunoglobulin superfamily, predominantly found on macrophages and monocytes (1-4). CD64 has a high affinity for the antibody-constant region of IgG, with three extracellular Ig-like domains (3). After binding IgG, CD64 interacts with an accessory chain known as the FcR common γ chain (FcRγ), which possesses an immunoreceptor tyrosine-based activation motif (ITAM) that is necessary for triggering cellular activation (2,3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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