WB, IHC-P
M
Endogenous
60-100
Rabbit IgG
#P18337
20343
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #78881.
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with mouse CD62L/L-Selectin recombinant protein.
Background
L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).
Due to cell type-specific glycosylation, the actual molecular weight of L-Selectin differs between cell types and can range from 60 kDa in lymphocytes up to 100 kDa in neutrophils (7).
- Gallatin, W.M. et al. (1983) Nature 304, 30-4.
- Tedder, T.F. et al. (1989) J Exp Med 170, 123-33.
- Tedder, T.F. et al. (1990) J Immunol 144, 532-40.
- Berg, E.L. et al. (1991) J Cell Biol 114, 343-9.
- Finger, E.B. et al. (1996) Nature 379, 266-9.
- Sallusto, F. et al. (1999) Nature 401, 708-12.
- Ivetic, A. et al. (2019) Front Immunol 10, 1068.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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