WB, IHC-P
M
Endogenous
60-100
Rabbit IgG
#P18337
20343
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with mouse CD62L/L-Selectin recombinant protein.
Background
L-Selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).
Due to cell type-specific glycosylation, the actual molecular weight of L-Selectin differs between cell types and can range from 60 kDa in lymphocytes up to 100 kDa in neutrophils (7).
- Gallatin, W.M. et al. (1983) Nature 304, 30-4.
- Tedder, T.F. et al. (1989) J Exp Med 170, 123-33.
- Tedder, T.F. et al. (1990) J Immunol 144, 532-40.
- Berg, E.L. et al. (1991) J Cell Biol 114, 343-9.
- Finger, E.B. et al. (1996) Nature 379, 266-9.
- Sallusto, F. et al. (1999) Nature 401, 708-12.
- Ivetic, A. et al. (2019) Front Immunol 10, 1068.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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