WB, W-S, IP
H
Endogenous
70-80
Rabbit
#P20138
945
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD33 protein. Antibodies are purified by peptide affinity chromatography.
Background
CD33, a type I transmembrane protein, is a sialic acid-binding Ig-like lectin (Siglec-3) of the Ig superfamily, and human CD33 binds preferentially to alpha-2, 6-linked sialic acid. Upon binding to its ligands CD33 transduces an inhibitory signaling through the immunoreceptor tyrosine-based inhibitory motif (ITIM) in its intracellular domain, inhibiting cellular function such as phagocytosis. In addition, CD33 is also involved in other processes, such as adhesion (1-3). Due to its exclusive expression on hematopoietic cells, particularly the myeloid lineage and their progenitors, CD33 has been actively pursued as a therapeutic target against acute myeloid leukemia (AML) (4,5). CD33 may also be involved in Alzheimer’s Disease (6-8).
- Avril, T. et al. (2004) J Immunol 173, 6841-9.
- Cao, H. and Crocker, P.R. (2011) Immunology 132, 18-26.
- Crocker, P.R. et al. (2012) Ann N Y Acad Sci 1253, 102-11.
- Laszlo, G.S. et al. (2014) Blood Rev 28, 143-53.
- Laing, A.A. et al. (2017) Exp Hematol 54, 40-50.
- Jiang, T. et al. (2014) Mol Neurobiol 49, 529-35.
- Siddiqui, S.S. et al. (2017) J Biol Chem 292, 15312-20.
- Zhao, L. (2018) Gerontology, 1-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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