Revision 5

#48893

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IHC-P, IF-IC

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
170

Source/Isotype:
Rabbit IgG

UniProt ID:
#P11215

Entrez-Gene Id:
3684

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunohistochemistry (Paraffin) 1:100 - 1:400
Immunofluorescence (Immunocytochemistry) 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #90390.

Specificity/Sensitivity

CD11b/ITGAM (E3J2F) Rabbit mAb recognizes endogenous levels of total CD11b/ITGAM protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD11b/ITGAM protein.

Background

Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CD11b/ITGAM (E3J2F) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of CD11b/ITGAM protein in A549 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: CD11b/ITGAM (E3J2F) Rabbit mAb
Simple Western™ analysis of lysates (1.0 mg/mL) from THP-1 cells using CD11b/ITGAM (E3J2F) Rabbit mAb #48893. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
Western Blotting Image 1: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 1: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 2: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 3: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 4: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 5: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 6: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 7: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 8: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 9: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human brain using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 10: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human tonsil using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 11: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human small intestine using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 12: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human liver using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 13: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human colon using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 14: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human spleen using CD11b/ITGAM (E3J2F) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 15: CD11b/ITGAM (E3J2F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded THP-1 cell pellet (left, positive) or Jurkat cell pellet (right, negative) using CD11b/ITGAM (E3J2F) Rabbit mAb.
Immunohistochemistry Image 16: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of THP-1 cells (left, positive) and A549 cells (right, negative) using CD11b/ITGAM (E3J2F) Rabbit mAb (green) and DAPI #4083 (blue). THP-1 cells were treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 18 hr) to promote adherence for imaging.
Immunofluorescence Image 1: CD11b/ITGAM (E3J2F) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.