WB, IP, IHC-P
H
Endogenous
160
Rabbit IgG
#P20701
3683
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #36769.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val975 of human CD11α protein.
Background
CD11α, also known as integrin alpha-L (ITGAL), is the alpha chain of the lymphocyte function-associated antigen-1 (LFA-1). CD11α combines with the beta chain CD18 (ITGB2) to form LFA-1, an integrin expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands ICAM-1, 2, 3, and 4 (1), F11R/JAM-1 (2,3), and the secreted form of ubiquitin-like protein ISG15 (4). LFA-1 is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction and transmigration (2,3), cytotoxic T cell co-stimulation (5), cytotoxic NK cell mediated killing (6), leukocyte clearance (7), and antibody-dependent killing by granulocytes and monocytes (8,9). LFA-1 is required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (10). High expression of CD11α has been associated with antigen-specific and tumor-reactive T cells (11,12).
- Hynes, R.O. (1992) Cell 69, 11-25.
- Ostermann, G. et al. (2002) Nat Immunol 3, 151-8.
- Fraemohs, L. et al. (2004) J Immunol 173, 6259-64.
- Swaim, C.D. et al. (2017) Mol Cell 68, 581-590.e5.
- Ni, H.T. et al. (2001) J Immunol 166, 6523-9.
- Barber, D.F. et al. (2004) J Immunol 173, 3653-9.
- Kristóf, E. et al. (2013) Apoptosis 18, 1235-51.
- Springer, T.A. et al. (1985) Adv Exp Med Biol 184, 311-22.
- Kohl, S. et al. (1984) J Immunol 133, 2972-8.
- Bose, T.O. et al. (2014) J Immunol 193, 2863-72.
- Liu, X. et al. (2013) Oncoimmunology 2, e23972.
- Christiaansen, A.F. et al. (2017) Vaccine 35, 4255-61.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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