WB, IP, IHC-P, IF-IC, FC-FP
H
Endogenous
45, 55
Rabbit IgG
#P13598
3384
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:100 |
Immunofluorescence (Immunocytochemistry) | 1:100 |
Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CD102 (ICAM-2) protein.
Background
Intercellular cell adhesion molecule-2 (CD102/ICAM-2) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. Like CD54/ICAM-1, CD102/ICAM-2 is a ligand that binds the leukocyte adhesion molecule LFA-1 (leukocyte function-associated antigen-1), which mediates intercellular interactions between immune cells and other cell types (1).
Expression of CD102/ICAM-2 has been shown to affect angiogenesis (2), cellular radioresistance (3) and anti-tumor immune response (4). Along with CD54/ICAM-1, CD102/ICAM-2 mediates T cell crawling and diapedesis across the blood-brain barrier (5), as well as T cell migration across the bronchial epithelium (6). CD102/ICAM-2 interaction with the actin cytoskeleton through α-actinin has been shown to limit the mobility on neuroblastoma cells (7), and this effect is dependent on glycosylation of CD102/ICAM-2 (8).
- Staunton, D.E. et al. (1989) Nature 339, 61-4.
- Huang, M.T. et al. (2005) Blood 106, 1636-43.
- Ishigami, T. et al. (2008) Br J Cancer 98, 1357-65.
- Hiraoka, N. et al. (2011) Gastroenterology 140, 310-21.
- Steiner, O. et al. (2010) J Immunol 185, 4846-55.
- Porter, J.C. and Hall, A. (2009) FASEB J 23, 492-502.
- Yoon, K.J. et al. (2008) PLoS One 3, e3629.
- Feduska, J.M. et al. (2013) BMC Cancer 13, 261.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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