WB
H
Endogenous
25, 37
Rabbit
#P25774
1520
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly233 of human cathepsin S protein. Antibodies are purified by peptide affinity chromatography.
Background
Cathepsin S (CTSS) is a cysteine protease belonging to the cathepsin family (1). It is synthesized as a 37 kDa pro-protein, which is proteolytically processed to remove the amino terminal activation peptide, yielding the mature (active) cathepsin S protein (2). The protein is localized to the lysosomal or endosomal compartments of antigen-presenting cells such as B cells, macrophages, and dendritic cells, where it plays an important role in immune response activation (3,4). Specifically, cathepsin S proteolytically removes the self-associated invariant chain (CD74) from MHC class II, and promotes MHC-exogenous antigenic peptide loading (5). Cathepsin S may also be secreted by cells to the extracellular matrix, where its proteolytic degradation of matrix proteins and cytokines (e.g., collagens, elastin, PAR2) has been shown to influence cell signaling (1). Aberrant expression and activity of cathepsin S have been associated with various disease states, including atherosclerosis, fibrosis and inflammation, autoimmune disease, and cancer (6-10), making it a potential biomarker and a promising therapeutic target.
- Wilkinson, R.D. et al. (2015) Biol Chem 396, 867-82.
- Huang, C.C. et al. (2016) Sci Rep 6, 29256.
- Villadangos, J.A. et al. (1999) Immunol Rev 172, 109-20.
- Nakagawa, T.Y. and Rudensky, A.Y. (1999) Immunol Rev 172, 121-9.
- Riese, R.J. et al. (1996) Immunity 4, 357-66.
- Wu, H. et al. (2018) J Atheroscler Thromb 25, 111-123.
- Kehlet, S.N. et al. (2017) BMC Pulm Med 17, 110.
- Sena, B.F. et al. (2017) Front Cardiovasc Med 4, 88.
- Clark, A.K. and Malcangio, M. (2012) Exp Neurol 234, 283-92.
- McDowell, S.H. et al. (2020) Biochim Biophys Acta Mol Cell Res 1867, 118781.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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