WB, IHC-P
H
Endogenous
29
Rabbit IgG
#P08311
1511
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro130 of human Cathepsin G protein.
Background
Cathepsin G is a serine protease which was first identified in the cytoplasmic azurophilic granules of neutrophil leukocytes (1). As a degradative enzyme, Cathepsin G acts both intracellularly to degrade ingested host pathogens and extracellularly to breakdown ECM components at inflammatory sites (2). Independent of its serine protease activity, Cathepsin G is also known to exert broad-spectrum antibacterial action against Gram-negative and –positive bacteria (3). Cathepsin G has been reported to play an important role in the pathophysiology of several diseases, such as chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, cystic fibrosis, and other conditions clinically manifested by excessive inflammatory reactions (4-7). Because of its role in host defense and disease, Cathepsin G is of interest as a potential therapeutic target (8,9).
- Starkey, P.M. and Barrett, A.J. (1976) Biochem J 155, 273-8.
- Pham, C.T. (2006) Nat Rev Immunol 6, 541-50.
- Shafer, W.M. et al. (1991) J Biol Chem 266, 112-6.
- Gudmann, N.S. et al. (2018) Biochem Biophys Res Commun 503, 1284-90.
- Nordstrom, D. et al. (1996) Clin Rheumatol 15, 35-41.
- Twigg, M.S. et al. (2015) Mediators Inflamm 2015, 293053.
- Shimoda, N. et al. (2007) Am J Pathol 170, 930-40.
- Korkmaz, B. et al. (2010) Pharmacol Rev 62, 726-59.
- Alatrash, G. et al. (2017) Leukemia 31, 234-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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