Revision 3

#88239

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-F, IF-IC

Reactivity:
M

Sensitivity:
Endogenous

MW (kDa):
46, 43, 28

Source/Isotype:
Rabbit IgG

UniProt ID:
#P18242

Entrez-Gene Id:
13033

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:500 - 1:2000
Immunofluorescence (Frozen) 1:50 - 1:200
Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #52899.

Specificity/Sensitivity

Cathepsin D (E7Z4L) XP® Rabbit mAb recognizes endogenous levels of preprocathepsin D, procathepsin D and the heavy and light chain subunit of mature cathepsin D protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse cathepsin D protein.

Background

Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。

专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 , (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines and mouse brain tissue using Cathepsin D (E7Z4L) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse liver using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemistry Image 1: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse kidney using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemistry Image 2: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse lung using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemistry Image 3: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemistry Image 4: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse thymus using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemistry Image 5: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using Cathepsin D (E7Z4L) XP® Rabbit mAb.

Immunohistochemistry Image 6: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using Cathepsin D (E7Z4L) XP® Rabbit mAb.
Immunohistochemistry Image 7: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse colon using Cathepsin D (E7Z4L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 8: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen wild-type mouse brain using Cathepsin D (E7Z4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (magenta pseudocolor).
Immunofluorescence Image 1: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer's disease using Cathepsin D (E7Z4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (magenta pseudocolor).
Immunofluorescence Image 2: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of fixed frozen wild-type mouse brain (left) or brain from an amyloid mouse model of Alzheimer's disease (right) using Cathepsin D (E7Z4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (red), GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (cyan pseudocolor), and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #35363 (blue pseudocolor).
Immunofluorescence Image 3: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Projected confocal z-stack of fixed frozen mouse kidney using Cathepsin D (E7Z4L) XP® Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #20825 (green) and DAPI #4083 (blue).
Immunofluorescence Image 4: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Confocal immunofluorescent analysis of NIH/3T3 cells using Cathepsin D (E7Z4L) XP® Rabbit mAb (green) and DAPI #4083 (blue).
Immunofluorescence Image 1: Cathepsin D (E7Z4L) XP<sup>®</sup> Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.