Revision 5
#74089
Store at -20C
Orders:
877-616-CELL (2355)
Support:
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, W-S, IHC-P, IF-F, IF-IC
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
46, 43, 28
Source/Isotype:
Rabbit IgG
UniProt ID:
#P18242
Entrez-Gene Id:
13033
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunohistochemistry (Paraffin) | 1:75 - 1:300 |
| Immunofluorescence (Frozen) | 1:800 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity
Cathepsin D (E5V4H) Rabbit mAb detects endogenous levels of preprocathepsin D, procathepsin D, and the heavy chain subunit of mature cathepsin D protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu179 of mouse cathepsin D protein.
Background
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9).
Background References
- Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4910-4.
- Erickson, A.H. et al. (1981) J. Biol .Chem. 256, 11224-31.
- Liaudet-Coopman, E. et al. (2006) Cancer Lett. 237, 167-79.
- Berchem, G. et al. (2002) Oncogene 21, 5951-5.
- Nomura, T. and Katunuma, N. (2005) J. Med. Invest. 52, 1-9.
- Garcia, M. et al. (1996) Stem Cells 14, 642-50.
- Nogami, M. et al. (2000) Histochem. J. 32, 505-8.
- Nakanishi, H. (2003) Ageing Res. Rev. 2, 367-81.
- Callahan, L.M. et al. (1998) Neurobiol. Aging 19, S99-105.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting W-S: Simple Western™ IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse R: Rat
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Western blot analysis of extracts from various brain tissues and HCC1419 cells using Cathepsin D (E5V4H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Simple Western™ analysis of lysates (0.5 mg/ml) from mouse brain lysates using Cathepsin D (E5V4H) Rabbit mAb #74089. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230kDa.
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using Cathepsin D (E5V4H) Rabbit mAb.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human adrenal gland using Cathepsin D (E5V4H) Rabbit mAb.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Immunohistochemical analysis of paraffin-embedded normal human pancreas using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human lymph node using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human brain using Cathepsin D (E5V4H) Rabbit mAb.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse thymus using Cathepsin D (E5V4H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse testes using Cathepsin D (E5V4H) Rabbit mAb.
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma (left) and tonsil (right) using Cathepsin D (E5V4H) Rabbit mAb (top) or a Cathepsin D Rabbit pAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human cathepsin D. The similar patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum (left) and A20 syngeneic tumor (right) using Cathepsin D (E5V4H) Rabbit mAb (top) or a Cathepsin D Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on mouse cathepsin D. The similar patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded normal human liver using Cathepsin D (E5V4H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Immunohistochemical analysis of paraffin-embedded U-118 MG cell pellet (left, positive) or Ramos cell pellet (right, negative) using Cathepsin D (E5V4H) Rabbit mAb.
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer's disease using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and β-Amyloid (D3D2N) Mouse mAb #15126 (right, blue).
Confocal immunofluorescent analysis of fixed frozen mouse brain using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.
Revision 5
Confocal immunofluorescent analysis of fixed frozen mouse colon at high magnification using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Confocal immunofluorescent analysis of fixed frozen mouse colon at low magnification using Cathepsin D (E5V4H) Rabbit mAb (left, green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #36618 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Confocal immunofluorescent analysis of HCC1419 cells (left) and U-118 MG cells (right) using Cathepsin D (E5V4H) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue).
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] •
Web:
cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.