WB
H
Endogenous
28-30
Rabbit IgG
#P31944
23581
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human caspase-14 protein.
Background
Caspases are a family of cysteine proteases that play an essential role in carrying out apoptosis. Caspase-14, also named MICE, is a unique member of the caspase family with restricted expression; it is found in embryonic tissues and adult skin (1,2). Caspase-14 is weakly processed into p18 and p11 subunits by caspase-8 (2). Caspase-14 may not play a role in apoptosis (2), but instead may regulate keratinocyte differentiation (3). Expression of caspase-14 may protect from psoriasis (4,5) and irradiation damage (6). Caspase-14 may also be responsible for proteolytic processing of filaggrin during terminal differentiation of keratinocytes (6).
- Hu, S. et al. (1998) J Biol Chem 273, 29648-53.
- Van de Craen, M. et al. (1998) Cell Death Differ 5, 838-46.
- Eckhart, L. et al. (2000) Biochem Biophys Res Commun 277, 655-9.
- Lippens, S. et al. (2004) Am J Pathol 165, 833-41.
- Walsh, D.S. et al. (2005) J Dermatol Sci 37, 61-3.
- Denecker, G. et al. (2007) Nat Cell Biol 9, 666-74.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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