Revision 1

#62114

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
33

Source/Isotype:
Rabbit IgG

UniProt ID:
#P52907

Entrez-Gene Id:
829

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

CAPZA1 (E3U1G) Rabbit mAb recognizes endogenous levels of total CAPZA1 protein. This antibody does not cross-react with CAPZA2 protein. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with CAPZA3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg86 of human CAPZA1 protein.

Background

The F-actin-capping protein, a ubiquitously expressed heterodimer composed of structurally similar α and β subunits, regulates cytoskeletal organization and function by binding to the actin filament barbed ends and maintaining the cellular pool of monomeric actin (1).

F-actin-capping protein subunit α-1 (CAPZA1) overexpression is linked to more favorable prognosis in gastric cancer (2). In hepatocellular carcinoma, CAPZA1 inhibits epithelial-mesenchymal transition (EMT), migration, and invasion (3,4).  

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from 293 cells, transfected with control siRNA (-) or CAPZA1 siRNA (+), using CAPZA1 (E3U1G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 1: CAPZA1 (E3U1G) Rabbit mAb
Western blot analysis of extracts from various cell lines using CAPZA1 (E3U1G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: CAPZA1 (E3U1G) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.