WB, IP
H M R
Endogenous
42
Rabbit
#Q14012
8536
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CaMKI-α protein. Antibodies are purified by peptide affinity chromatography.
Background
The Ca2+/calmodulin-dependent kinase (CaMK) family, which is activated in response to elevation of intracellular Ca2+, includes CaMKI, CaMKII, CaMKIV, and CaMK-kinases (CaMKKs) (1,2). CaMKI is a downstream substrate of CaMKK and has 4 isoforms: CaMKI-α, CaMKI-β, CaMKI-γ, and CaMKI-δ. CaMKI is present in most cell types and may be involved in cellular functions, including transcription, cytoskeletal organization, axonal growth cone motility, and long-term potentiation in neurons (3-6). CaMKII is also ubiquitously expressed in most cell types. While muscular CaMKII has been linked to activation of mitochondrial biogenesis in muscle hypertrophy response, neuronal CaMKII regulates important neuronal functions, including neurotransmitter synthesis, neurotransmitter release, modulation of ion channel activity, cellular transport, cell morphology and neurite extension, synaptic plasticity, learning and memory, and gene expression (7). Like CaMKI, CaMKIV is also a substrate of CaMKKs and is primarily restricted to the nucleus of neurons. CaMKIV regulates gene transcription in neurons through phosphorylation of transcription factors such as CREB and is required for fear memory (8).
- Chin, E.R. (2004) Proc. Nutr. Soc. 63, 279-286.
- Mizuno, K. and Giese, K.P. (2005) J. Pharmacol. Sci. 98, 191-197.
- Wayman, G.A. et al. (2004) J. Neurosci. 24, 3786-3794.
- Gardner, H.P. et al. (2000) Genomics 63, 279-288.
- Verploegen, S. et al. (2005) Blood 106, 1076-1083.
- Takemoto-Kimura, S. et al. (2003) J. Biol. Chem. 278, 18597-18605.
- Yamauchi, T. (2005) Biol. Pharm. Bull. 28, 1342-1354.
- Wei, F. et al. (2002) Nat. Neurosci. 5, 573-579.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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