WB
H M R Mk
Endogenous
70-80 non-muscle, 120-150 smooth muscle
Rabbit
#Q05682
800
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caldesmon-1. Antibodies are purified using peptide affinity chromatography.
Background
Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).
- Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126.
- Humphrey, M.B. et al. (1992) Gene 112, 197-204.
- Yamashiro, S. et al. (1991) Nature 349, 169-172.
- Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681.
- Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309.
- Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250.
- Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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