Revision 7

#43095

Store at -20C

C/EBPβ (E2K1U) Rabbit mAb
CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-IC, FC-FP

Reactivity:
H Mk

Sensitivity:
Endogenous

MW (kDa):
46 KD (LAP), 48 KD (Full Length)

Source/Isotype:
Rabbit IgG

UniProt ID:
#P17676

Entrez-Gene Id:
1051

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:400 - 1:1600
Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized) 1:3200 - 1:6400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #76191.

Specificity/Sensitivity

C/EBPβ (E2K1U) Rabbit mAb recognizes endogenous levels of total C/EBPβ protein. This antibody does not cross-react with C/EBPα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly67 of human C/EBPβ protein.

Background

C/EBPβ is a member of the transcription factor family of CCAAT/enhancer-binding proteins (C/EBPs) that are critical for cellular differentiation and function (1). There are various N-terminally truncated C/EBPβ isoforms: full-length C/EBPβ, C/EBPβ-LAP (liver-enriched activator protein), and C/EBPβ-LIP (liver-enriched inhibitory protein). In triple-negative breast cancer cells, aerobic glycolysis was shown to control the expression of C/EBPβ-LAP, which in turn stimulates the expression of granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). These factors are secreted from the cancer cells to promote myeloid-derived suppressor cell (MDSC) development in the tumor microenvironment, thereby suppressing anti-tumor immunity (2). In addition, research studies showed that decreased C/EBPβ-LIP expression delays age-related phenotypes in mice, suggesting a potential role for C/EBPβ-LIP in aging (3).

Background References

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: Human Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品 ， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#43095

C/EBPβ (E2K1U) Rabbit mAb

Cell Signaling Technology Logo
Western blot analysis of extracts from U-87 MG, A-172, and TT cells using C/EBPβ (E2K1U) Rabbit mAb (upper) and GAPDH (D16H11) XP® #5174 (lower). The higher expression of C/EBPβ in U-87 MG cells, lower expression of C/EBPβ in A-172 cells, and the absence of detectable C/EBPβ in TT cells is consistent with RNAseq data, confirming the specificity of the antibody for C/EBPβ.
Western Blotting Image 1: C/EBPβ (E2K1U) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using C/EBPβ (E2K1U) Rabbit mAb.
Immunohistochemistry Image 1: C/EBPβ (E2K1U) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using C/EBPβ (E2K1U) Rabbit mAb.
Immunohistochemistry Image 2: C/EBPβ (E2K1U) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#43095

C/EBPβ (E2K1U) Rabbit mAb

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using C/EBPβ (E2K1U) Rabbit mAb.
Immunohistochemistry Image 3: C/EBPβ (E2K1U) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using C/EBPβ (E2K1U) Rabbit mAb.
Immunohistochemistry Image 4: C/EBPβ (E2K1U) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human skin using C/EBPβ (E2K1U) Rabbit mAb.
Immunohistochemistry Image 5: C/EBPβ (E2K1U) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#43095

C/EBPβ (E2K1U) Rabbit mAb

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human placenta using C/EBPβ (E2K1U) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). 
Immunohistochemistry Image 6: C/EBPβ (E2K1U) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded U-87 MG cell pellet (left, positive) or TT cell pellet (right, negative) using C/EBPβ (E2K1U) Rabbit mAb.
Immunohistochemistry Image 7: C/EBPβ (E2K1U) Rabbit mAb
Confocal immunofluorescent analysis of U-87 MG cells (left, high-expressing), A-172 cells (middle, low-expressing), and TT cells (right, negative) using C/EBPβ (E2K1U) Rabbit mAb (green), DyLight™ 554 Phalloidin #13054 (red), and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: C/EBPβ (E2K1U) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 7

#43095

C/EBPβ (E2K1U) Rabbit mAb

Cell Signaling Technology Logo
Flow cytometric analysis of TT cells (blue, negative) and U-87 MG cells (green, positive) using C/EBPβ (E2K1U) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: C/EBPβ (E2K1U) Rabbit mAb
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.