Revision 3
#94032
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, ChIP, ChIP-seq
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
100
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q15059
Entrez-Gene Id:
8019
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Background References
- Belkina, A.C. and Denis, G.V. (2012) Nat Rev Cancer 12, 465-77.
- Voigt, P. and Reinberg, D. (2011) Genome Biol 12, 133.
- Wu, S.Y. and Chiang, C.M. (2007) J Biol Chem 282, 13141-5.
- LeRoy, G. et al. (2008) Mol Cell 30, 51-60.
- Lamonica, J.M. et al. (2011) Proc Natl Acad Sci USA 108, E159-68.
- French, C.A. et al. (2008) Oncogene 27, 2237-42.
- Dawson, M.A. et al. (2011) Nature 478, 529-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
W: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq
Cross-Reactivity Key
H: Human Mk: Monkey
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
限制使用
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Revision 3
#94032
BRD3 (E3D5N) Rabbit mAb
Revision 3
#94032
BRD3 (E3D5N) Rabbit mAb
Chromatin immunoprecipitations were performed with cross-linked chromatin from MV-4-11 cells and BRD3 (E3D5N) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across Myc, a known target gene of BRD3 (see additional figure containing ChIP-qPCR data).
Chromatin immunoprecipitations were performed with cross-linked chromatin from MV-4-11 cells and either BRD3 (E3D5N) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human c-Myc promoter primers, human BCL2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
