WB, IP
H
Endogenous
55-65
Rabbit IgG
#P22004
654
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln173 of human BMP6 protein.
Background
Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belong to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the C-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5, and 8), resulting in the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).
BMP6 plays a critical role in iron metabolism, ovulation, and aldosterone production (5-7). Recent studies have shown that hypermethylation of the BMP6 gene promoter decreases its expression in several cancer types, which contributes to tumor growth and progression (8-11).
- Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
- Wozney, J.M. et al. (1988) Science 242, 1528-34.
- Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
- Nohe, A. et al. (2004) Cell Signal 16, 291-9.
- Roth, M.P. and Coppin, H. (2009) Med Sci (Paris) 25, 678-80.
- Inagaki, K. et al. (2006) Endocrinology 147, 2681-9.
- Akiyama, I. et al. (2014) Reprod Sci 21, 772-7.
- Hashida, Y. et al. (2012) Oncol Rep 27, 825-30.
- Liu, G. et al. (2014) Oncol Rep 32, 581-8.
- He, Y. et al. (2014) PLoS One 9, e87994.
- Sangplod, P. et al. (2014) Asian Pac J Cancer Prev 15, 7091-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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