WB, IP, IF-IC
H
Endogenous
70
Rabbit IgG
#Q15582
7045
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunofluorescence (Immunocytochemistry) | 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human βIG-H3 protein.
Background
βIG-H3 (TGFBI/RGD-CAP/Kerato-epithelin) is a 683-amino acid secretory protein induced by TGF-β that plays a role in cell adhesion, differentiation, and apoptosis (1-4). βIG-H3 contains an internal cysteine-rich EMI domain followed by four fasciclin-1 domains and a carboxy terminal RGD domain (1,2). It contributes to cell adhesion through interactions with integrins as well as a number of extracellular matrix (ECM) proteins including collagen, fibronectin, and laminin (5-7). ECM βIG-H3 is found in a wide variety of tissues (8-12). Mutations in the βIG-H3 gene as well as elevated protein levels are most notably associated with corneal dystrophies (13).
- Skonier, J. et al. (1992) DNA Cell Biol 11, 511-22.
- Skonier, J. et al. (1994) DNA Cell Biol 13, 571-84.
- Hashimoto, K. et al. (1997) Biochim Biophys Acta 1355, 303-14.
- Kim, J.E. et al. (2003) Oncogene 22, 2045-53.
- Kim, J.E. et al. (2002) Invest Ophthalmol Vis Sci 43, 656-61.
- Billings, P.C. et al. (2002) J Biol Chem 277, 28003-9.
- Hanssen, E. et al. (2003) J Biol Chem 278, 24334-41.
- Gibson, M.A. et al. (1997) J Histochem Cytochem 45, 1683-96.
- Billings, P.C. et al. (2000) Am J Respir Cell Mol Biol 22, 352-9.
- Gilbert, R.E. et al. (1998) Kidney Int 54, 1052-62.
- Rawe, I.M. et al. (1997) Invest Ophthalmol Vis Sci 38, 893-900.
- LeBaron, R.G. et al. (1995) J Invest Dermatol 104, 844-9.
- Munier, F.L. et al. (1997) Nat Genet 15, 247-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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