B7-H6 (E9B2L) Rabbit mAb (#65566) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

REACTIVITY:

SENSITIVITY:

Endogenous

MW (kDa):

80-90

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q68D85

Entrez-Gene Id:

374383

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

B7-H6 (E9B2L) Rabbit mAb recognizes endogenous levels of total B7-H6 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human B7-H6 recombinant protein.

Background

B7-H6 (NCR3LG1) is a member of the B7 family of proteins which provide costimulatory signals for regulating T cell responses (1-3). B7-H6 is a ligand for the NK-cell–activating receptor NKp30, which triggers anti-tumor natural killer (NK) cell cytotoxicity and cytokine secretion (1). Both forms of B7-H6, cell surface B7-H6 and soluble B7-H6 (sB7-H6), are detected in primary tumors and the tumor microenvironment, but rarely in normal tissues (1-5). Cell surface B7-H6 associates with cell damage, and triggers innate immunity when it binds to NKp30 on NK cells (3,5). Soluble B7-H6 can decrease NKp30 expression on NK cells and induce NK cell dysfunction (4,5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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