IHC-Bond, IHC-P
H
Endogenous
Mouse IgM
#Q9P2W7
27087
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with B3GAT1 expressing human peripheral blood mononuclear cells.
Background
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1), also known as beta-1,3-glucuronyltransferase 1 and glucuronosyltransferase P, is a type II membrane protein enzyme encoded by the B3GAT1 gene. This gene is localized on the 11q25 chromosome and is a member of the glucuronyltransferase gene family (1). B3GAT1 is one of the enzymes involved in the biosynthesis of carbohydrate epitope human natural killer-1 (HNK-1), also known as CD57 and Leu7 (2). B3GAT1 catalyzes the glucuronyl transfer reaction that adds a glucuronic acid to the terminal N-acetyllactosamine (Lac) disaccharide to form the CD57 epitope on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (3). CD57 is present on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, as well as neural cells and striated muscle (4-7). The CD57 epitope may play a role in neural cell adhesion (8), and characterizes unique maturation states in T and NK cells (9,10).
- Mitsumoto, Y. et al. (2000) Genomics 65, 166-73.
- Abo, T. and Balch, C.M. (1981) J Immunol 127, 1024-9.
- Górska, A. et al. (2016) Arch Immunol Ther Exp (Warsz) 64, 497-503.
- Kared, H. et al. (2016) Cancer Immunol Immunother 65, 441-52.
- Lopez-Vergès, S. et al. (2010) Blood 116, 3865-74.
- Focosi, D. et al. (2010) J Leukoc Biol 87, 107-16.
- Mechtersheimer, G. et al. (1991) Cancer Res 51, 1300-7.
- Künemund, V. et al. (1988) J Cell Biol 106, 213-23.
- Sze, D.M. et al. (2001) Blood 98, 2817-27.
- Nielsen, C.M. et al. (2013) Front Immunol 4, 422.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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