WB
M R
Endogenous
28
Rabbit
#Q9WVJ5
12960
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu244 of mouse β-crystallin B1 protein. Antibodies are purified by peptide affinity chromatography.
Background
β-crystallin B1, encoded by crybb1, is a member of the β-crystallins, one of three major crystallin types encoded by the mammalian genome, which also includes alpha- and gamma-crystallins. Crystallins are expressed at very high levels in the retinal lens and form homo/heterodimers via its N-terminal arms (1). Crystallins generally function to maintain the transparency and refractive power of the eye lens. However, they are expressed outside of the lens in other tissues and have alternative non-lens functions (2). For example, Nuc1 mutant rat, in which the βA3/A-crystallin gene is mutated, exhibit vascular remodeling abnormalities via an astrocyte-dependent mechanism (3,4). The precise cellular function of β-crystallins in glia is not well understood, but may involve regulation of endolysosomal acidification, Golgi trafficking, and specific forms of cell death (5,6). Interestingly, crybb1, is highly enriched in microglia outside of the mammalian lens and may serve as a distinct marker for microglia (7).
- Van Montfort, R.L. et al. (2003) Protein Sci 12, 2606-12.
- Slingsby, C. and Wistow, G.J. (2014) Prog Biophys Mol Biol 115, 52-67.
- Sinha, D. et al. (2005) Exp Eye Res 80, 323-35.
- Sinha, D. et al. (2012) Transgenic Res 21, 1033-42.
- Ma, B. et al. (2011) Cell Death Dis 2, e217.
- Valapala, M. et al. (2013) Nat Commun 4, 1629.
- Crotti, A. and Ransohoff, R.M. (2016) Immunity 44, 505-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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