WB, IP, ChIP
H
Endogenous
280
Rabbit IgG
#P46100
546
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1189 of human ATRX protein.
Background
α-thalassemia/mental retardation X-linked (ATRX) is a transcriptional regulator and helicase that belongs to the SNF2 family of chromatin remodeling proteins (1,2). Together with its binding partner death-associated protein 6 (Daxx), ATRX acts as histone chaperone to deposit histone variant H3.3 at repetitive DNA sequences such as telomeric, pericentric, and ribosomal gene repeats (3-6). ATRX is involved in many nuclear functions that ensure proper sister chromatid cohesion during mitosis and chromosome alignment during meiosis (7,8). The ATRX transcriptional regulator also plays a role in the maintenance of telomere integrity and the regulation of gene expression during mammalian development by influencing DNA methylation patterns at high DNA repeat sequences (9,10). Mutations in the corresponding ATRX gene results in ATR-X syndrome, an X-linked disorder characterized by intellectual disabilities, craniofacial abnormalities, and mild α-thalassemia (11,12). Research studies indicate that the loss of ATRX protein occurs in numerous cancers, including pancreatic neuroendocrine tumors (PanNETs) and pediatric glioblastoma, where telomere maintenance occurs independently of telomerase (13-16).
- Clynes, D. et al. (2013) Trends Biochem Sci 38, 461-6.
- Picketts, D.J. et al. (1996) Hum Mol Genet 5, 1899-907.
- Drané, P. et al. (2010) Genes Dev 24, 1253-65.
- Elsässer, S.J. et al. (2012) Nature 491, 560-5.
- Lewis, P.W. et al. (2010) Proc Natl Acad Sci U S A 107, 14075-80.
- Goldberg, A.D. et al. (2010) Cell 140, 678-91.
- Ritchie, K. et al. (2008) J Cell Biol 180, 315-24.
- De La Fuente, R. et al. (2004) Dev Biol 272, 1-14.
- Wong, L.H. et al. (2010) Genome Res 20, 351-60.
- Gibbons, R.J. et al. (2000) Nat Genet 24, 368-71.
- Gibbons, R.J. et al. (1995) Cell 80, 837-45.
- Gibbons, R.J. et al. (1995) Hum Mol Genet 4 Spec No, 1705-9.
- Heaphy, C.M. et al. (2011) Science 333, 425.
- Lovejoy, C.A. et al. (2012) PLoS Genet 8, e1002772.
- Schwartzentruber, J. et al. (2012) Nature 482, 226-31.
- Jiao, Y. et al. (2011) Science 331, 1199-203.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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