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53217
PhosphoPlus® ATR (Thr1989) Antibody Duet
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PhosphoPlus® ATR (Thr1989) Antibody Duet #53217

Citations (1)
Simple Western™ analysis of lysates (0.1 mg/mL) from HeLa + Hydroxyurea (1.5mM, 17hr) cells using Phospho-ATR (Thr1989) (D5K8W) Rabbit mAb #30632. The virtual lane view (left) shows a single target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
Western blot analysis of extracts from various cell lines using ATR (E1S3S) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with hydroxyurea (1.5 mm, 16 hr; +), using Phospho-ATR (Thr1989) (D5K8W) Rabbit mAb (upper) or ATR (E1S3S) Rabbit mAb #13934 (lower).
Immunoprecipitation of ATR from HT-29 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ATR (E1S3S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using ATR Antibody #2790.
To Purchase # 53217S
Cat. # Size Price Inventory
53217S
1 Kit

Product Includes Quantity Reactivity MW(kDa) Isotype
ATR (E1S3S) Rabbit mAb 13934 100 µl H M R 300 Rabbit IgG
Phospho-ATR (Thr1989) (D5K8W) Rabbit mAb 30632 100 µl H 300 Rabbit IgG

Kit Usage Information

Protocols

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. ATR was long thought to exist in a constitutively active state in cells, with DNA damage-induced signaling occurring via recruitment of ATR to single stranded DNA and sites of replication stress. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR (4,5). Recent work has shown autophosphorylation of ATR at threonine 1989. Like ATM Ser1981, phosphorylation of ATR Thr1989 occurs in response to DNA damage, indicating that phosphorylation at this site is important in ATR-mediated signaling (6,7).

Pathways

Explore pathways related to this product.

Limited Uses

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