WB, IP
H Mk
Endogenous
19
Rabbit
#Q9NVP2
55723
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding the the carboxy-terminus of the human ASF1B protein.
Background
ASF1 was first identified in S. cerevisiae based on its ability to de-repress transcriptional silencing when overexpressed (1). While only one gene exists in yeast and Drosophila, mammalian cells contain the two highly homologous ASF1A and ASF1B genes (2). ASF1A and ASF1B function as histone chaperones, delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA (2-5). Both ASF1A and ASF1B bind to CAF-1, but only ASF1A binds to HIRA (5). In addition to playing a role in DNA replication and gene silencing, ASF1 functions in DNA damage repair, genome stability and cellular senescence. Deletion of ASF1 in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint (6-8). Depletion of both ASF1A and ASF1B in mammalian cells results in the accumulation of cells in S phase, increased phosphorylation of H2A.X, centrosome amplification and apoptosis (9,10). ASF1A is required for the formation of senescence-associated heterochromatin foci (SAHF), with overexpression of ASF1A inducing senescence in primary cells (4). Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress (11,12). The function of ASF1 phosphorylation is not yet understood.
- Singer, M.S. et al. (1998) Genetics 150, 613-632.
- Mousson, F. et al. (2007) Chromosoma 116, 79-93.
- Tang, Y. et al. (2006) Nat. Struct. Mol. Biol. 13, 921-929.
- Zhang, R. et al. (2005) Dev. Cell. 8, 19-30.
- Daganzo, S.M. et al. (2003) Curr. Biol. 13, 2148-2158.
- Ramey, C.J. et al. (2004) Mol. Cell. Biol. 24, 10313-10327.
- Prado, F. et al. (2004) EMBO Rep. 5, 497-502.
- Tyler, J.K. et al. (1999) Nature 402, 555-560.
- Sanematsu, F. et al. (2006) J. Biol. Chem. 281, 13817-13827.
- Groth, A. et al. (2005) Mol. Cell. 17, 301-311.
- Silljé, H.H. and Nigg, E.A. (2001) Curr. Biol. 11, 1068-1073.
- Carrera, P. et al. (2003) Genes Dev. 17, 2578-2590.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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