WB, IP
H
Endogenous
135
Rabbit IgG
#O00203
8546
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human AP3B1 protein.
Background
The adaptor protein complex 3 (AP-3) is a ubiquitously expressed, heterotetramer that is comprised of two large adaptins (AP3D1 and either AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2), and a small adaptin (AP3S1 or AP3S2). The AP-3 complex is involved in the sorting of protein cargo within the endosomal compartment that are destined for lysosomes (1,2). Defects in the AP-3 complex have been associated with Hermansky-Pudlak syndrome (HPS), a genetic disorder characterized by abnormal skin pigmentation, blood clotting abnormalities, and pulmonary fibrosis. Mutations in the gene encoding ubiquitously expressed AP3B1/β3A, destabilize the protein and the entire AP-3 complex, which results in defective trafficking of lysosomal membrane proteins (3,4). Research studies have shown that certain viral proteins have evolved to co-opt the AP-3 complex by binding to AP3B1 in order to facilitate viral particle assembly (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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