WB
H M R Mk
Endogenous
48
Rabbit
#P05549
7020
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human AP-2α. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The sequence-specific transcription factor activator protein 2α (AP-2α) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2α expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2α expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2α expression. Furthermore, p53 binds to the cis-element in the AP-2α promoter, suggesting that AP-2α is a target of p53. AP-2α may mediate the effect of p53 to inhibit cell proliferation (8).
- Mitchell, P.J. et al. (1987) Cell 50, 847-61.
- Williams, T. and Tjian, R. (1991) Genes Dev 5, 670-82.
- Douglas, D.B. et al. (2004) Cancer Res 64, 1611-20.
- Gee, J.M. et al. (1999) J Pathol 189, 514-20.
- Anttila, M.A. et al. (2000) Br J Cancer 82, 1974-83.
- Jean, D. et al. (1998) J Biol Chem 273, 16501-8.
- Ruiz, M. et al. (2004) Cancer Res 64, 631-8.
- Li, H. et al. (2006) Oncogene 25, 5405-15.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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