WB, IHC-P
H
Endogenous
28
Rabbit IgG
#P39687
8125
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:3000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human ANP32A protein.
Background
ANP32A is the founding member of the acidic nuclear phosphoprotein 32 kDa (ANP32) family, which share two highly conserved regions: the N-terminal leucine-rich repeats (LRRs) sequence and the C-terminal acidic tail (1). ANP32A was originally purified as a potent, heat-stable protein phosphatase 2A (PP2A)-specific inhibitor, and subsequent studies suggested that it binds directly to the catalytic subunit of PP2A (2,3). ANP32A is a key component of acetyltransferase inhibitor complex that regulates chromatin remodeling and transcription (4,5). ANP32A also forms a multiunit complex with HuR that regulates RNA transport and stability (6). In addition, ANP32A is reported to play roles in apoptosis, neural differentiation and pathogenesis (7-10).
- Matilla, A. and Radrizzani, M. (2005) Cerebellum 4, 7-18.
- Li, M. et al. (1995) Biochemistry 34, 1988-96.
- Li, M. et al. (1996) Biochemistry 35, 6998-7002.
- Seo, S.B. et al. (2002) J Biol Chem 277, 14005-10.
- Schneider, R. et al. (2004) J Biol Chem 279, 23859-62.
- Brennan, C.M. et al. (2000) J Cell Biol 151, 1-14.
- Jiang, X. et al. (2003) Science 299, 223-6.
- Chen, S. et al. (2008) J Biol Chem 283, 10513-21.
- Kular, R.K. et al. (2009) J Biol Chem 284, 7783-92.
- Cvetanovic, M. et al. (2012) Neurobiol Dis 48, 526-32.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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