WB
H M R
Endogenous
41
Mouse IgG1 kappa
#P09972
230
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly316 of human aldolase C protein.
Background
Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma (1). Furthermore, investigators have shown that changes in aldolase B gene expression levels have been observed in certain patients with this primary tumor (2,3).
Aldolase C is primarily expressed in the central nervous system (4). Studies have shown increased detection of serum aldolase C following traumatic brain injury (TBI) and mild TBI, showing potential for aldolase C as a biomarker for TBI (5,6).
- Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
- Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
- Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.
- Fujita, H. et al. (2014) PLoS One 9, e86679.
- Thelin, E.P. et al. (2018) Behav Brain Res 340, 71-80.
- Haddadi, K. et al. (2022) Iran J Med Sci 47, 33-39.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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