WB, IP
H Mk
Endogenous
130
Rabbit IgG
#Q8N4X5
84632
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly638 of human AFAP1L2/XB130 protein.
Background
The actin-filament associated protein (AFAP) family consists of AFAP1, AFAP1L1, and AFAP1L2/XB130, a group of structurally similar proteins that play distinct roles in the regulation of cytoskeletal dynamics and signal transduction. Actin filament-associated protein 1-like 2 (AFAP1L2, XB130) is an adaptor protein that regulates signaling downstream of multiple kinases, including Src, Akt, and the thyroid specific kinase RET/PTC (1-3). Through these pathways, AFAP1L2/XB130 mediates transcriptional regulation, cell proliferation, motility, and microRNA expression (4,5). Research has shown that AFAP1L2/XB130 is involved in the proliferation and survival of thyroid tumor cells (6), and may have value in gastric cancer prognosis (7).
- Xu, J. et al. (2007) J Biol Chem 282, 16401-12.
- Shiozaki, A. et al. (2012) PLoS One 7, e43646.
- Lodyga, M. et al. (2009) Oncogene 28, 937-49.
- Lodyga, M. et al. (2010) J Cell Sci 123, 4156-69.
- Takeshita, H. et al. (2013) PLoS One 8, e59057.
- Shiozaki, A. et al. (2011) Am J Pathol 178, 391-401.
- Shi, M. et al. (2012) PLoS One 7, e41660.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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