WB, IP
H M R Mk
Endogenous
100 pro-ADAM9-L, 80 ADAM9-L
Rabbit
#Q13443
8754
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human ADAM9. Antibodies are purified using protein A and peptide affinity chromatography.
Background
The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. Conserved domains in ADAM family members include a prodomain, a zinc-dependent metalloprotease domain, a disintegrin domain, a cysteine-rich domain, an EGF-like sequence, and a short cytoplasmic tail (1,2).
The prodomain is thought to aid in protein folding. Disintegrin and cysteine-rich domains mediate adhesion, at least in part, through binding to integrins. Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). ADAM9 is widely distributed and has been shown to affect migration in skin keratinocytes (3,4). Research studies have shown that ADAM9 is overexpressed in prostate cancer (5), pancreatic cancer (6), gastric cancer (7), and has been linked to invasion and metastasis in small cell lung cancer (8). Research has also shown that an alternatively spliced short (50 kDa) form of ADAM9 containing protease activity is involved in tumor cell invasion (9).
- N. M. Hooper and U. Lendeckel. . The Netherlands: Springer, 2005
- Schlöndorff, J. and Blobel, C.P. (1999) J Cell Sci 112 ( Pt 21), 3603-17.
- Franzke, C.W. et al. (2002) EMBO J 21, 5026-35.
- Zigrino, P. et al. (2007) J Biol Chem 282, 30785-93.
- Fritzsche, F.R. et al. (2008) Eur Urol 54, 1097-106.
- Grützmann, R. et al. (2004) Br J Cancer 90, 1053-8.
- Carl-McGrath, S. et al. (2005) Int J Oncol 26, 17-24.
- Shintani, Y. et al. (2004) Cancer Res 64, 4190-6.
- Mazzocca, A. et al. (2005) Cancer Res 65, 4728-38.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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