AceCS2/ACSS1 (E6T5E) Rabbit mAb (#37041) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, W-S

REACTIVITY:

H M

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#Q9NUB1

Entrez-Gene Id:

84532

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

AceCS2/ACSS1 (E6T5E) Rabbit mAb recognizes endogenous levels of total AceCS2/ACSS1 protein. This antibody does not cross-react with AceCS1/ACSS2 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly370 of human AceCS2/ACSS1 protein.

Background

Mitochondrial acetyl-CoA synthetase 2 (AceCS2), also known as acyl-CoA synthetase short-chain family member 1 (ACSS1), catalyzes acetyl-CoA biosynthesis from acetate and CoA. Studies show that acetate levels go up dramatically at sites of infection and inflammation, leading to the decrease of acetate assimilating enzymes AceCS2/ACSS1 and AceCS1/ACSS2 levels in memory CD8⁺ T cells. Acetate not metabolized by these enzymes instead stimulates glutaminase activity in memory CD8⁺ T cells, enhancing glutaminolysis, mitochondrial respiration, and cell survival (1). In addition, AceCS2/ACSS1 is highly expressed in one of the hepatocellular carcinoma (HCC) subtypes (2).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting W-S: Simple Western™

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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